human immortalized keratinocytes hacat cells  (Procell Inc)

Journal: International Journal of Molecular Sciences

Article Title: A Functional Evaluation of Resveratrol–Paclitaxel Combination Reveals Enhanced Apoptotic Responses in HeLa Cells

doi: 10.3390/ijms27104505

Figure Lengend Snippet: Limited selectivity in immortalized normal cells of RES and PAC in HeLa and HaCaT cells. ( A ) IC 50 values for RES in HeLa cervical cancer cells and HaCaT normal keratinocytes at 24 and 48 h. ( B ) IC 50 values for PAC under the same conditions. For both agents, IC 50 values were consistently higher in HaCaT cells compared to HeLa cells at both time points, indicating reduced sensitivity of normal cells. The selectivity index (SI), calculated as IC 50 (HaCaT)/IC 50 (HeLa), exceeded 2 in all conditions (RES: 2.6 and 2.2; PAC: 2.2 and 2.1 at 24 and 48 h, respectively), indicating limited selectivity in immortalized normal cells rather than definitive tumor-specific selectivity. Data are derived from three independent experiments ( n = 3). IC 50 values were calculated by nonlinear regression analysis of dose–response curves. It should be noted that HaCaT cells are immortalized keratinocytes and do not fully represent primary normal cervical epithelial cells.

Article Snippet: Human cervical carcinoma HeLa cells (ATCC ® CCL-2TM) and human immortalized keratinocyte HaCaT cells (Cell Lines Service, CLS, Cat. No. 300493, Eppelheim, Germany) were used in this study.

Techniques: Derivative Assay

Journal: Materials Today Bio

Article Title: Ultra-thin elastin-based membranes as an innovative dressing to enhance skin wound healing

doi: 10.1016/j.mtbio.2026.102898

Figure Lengend Snippet: Cell migration evaluation. A) Schematic illustration of the transwell migration assay showing cell seeding on the ELR membrane, migration through the membrane pores and transwell insert, and cell accumulation on the lower surface of the insert. Representative image of B) HFF-1 fibroblasts and C) HaCaT keratinocytes that migrated through the VKV-SKS membrane after 15 days of incubation. Experiments were performed using four independent samples (n = 4). D) A uniform gap size was achieved by employing a specific protocol involving the use of inserts. E) Quantitative analysis of the scratch wound recovery index (SWRI) widths at the indicated time points quantified by measuring the area of the scratched region. Data are shown as mean ± SEM with individual data points. Statistical analysis was performed using one-way ANOVA followed by Tukey's multiple-comparisons test. Significance levels reported as follows: ∗∗∗ (P < 0.001), ∗∗∗∗ (P < 0.0001). F) Representative photographs of the migration of serum-free keratinocytes in the presence of VKV-SKS membranes for wound closure at the indicated time points following the scratch. Scale bar = 200 μm.

Article Snippet: Biocompatibility was evaluated using two human cell lines: HFF-1 human foreskin fibroblasts (ATCC® SCRC-1041) and HaCaT immortalized human keratinocytes (CLS-300493).

Techniques: Migration, Transwell Migration Assay, Membrane, Incubation

Journal: Materials Today Bio

Article Title: Ultra-thin elastin-based membranes as an innovative dressing to enhance skin wound healing

doi: 10.1016/j.mtbio.2026.102898

Figure Lengend Snippet: Cytocompatibility and Cytotoxicity. A) Cell viability values obtained from an MTS assay conducted on HFF-1 and HaCaT cells seeded onto a VKV-SKS membrane and cultured for 48 h. Data are presented as mean ± SEM (n = 4 independent biological replicates). B) Effect of the ELR membrane on LDH release after 24 h of culturing. Data are presented as mean ± SEM (n = 4 independent biological replicates). C) Fluorescence microscope images of stained fibroblasts (HFF-1) and keratinocytes (HaCaT) cells, seeded onto VKV-SKS membranes, during a 21-day culture period. Green staining distinguished live cells, while red staining revealed cell death. A slight autofluorescence was detected on membranes in the green channel. Scale bar: 100 μm. D) Representative crystal violet staining images of cell culture on membranes for fibroblasts after 15 days of incubation. (E) Representative crystal violet staining images of cell culture on membranes for keratinocytes after 15 days of incubation. F) Number of colonies formed within ELR membranes. G) Plating efficiency refers to the ratio of the number of colonies to the number of cells seeded. Data are presented as mean ± SEM (n = 4 independent biological replicates). Significance levels reported as follows: ∗ (P < 0.05), ∗∗ (P < 0.01), ∗∗∗ (P < 0.001), and ∗∗∗∗ (P < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Biocompatibility was evaluated using two human cell lines: HFF-1 human foreskin fibroblasts (ATCC® SCRC-1041) and HaCaT immortalized human keratinocytes (CLS-300493).

Techniques: MTS Assay, Membrane, Cell Culture, Fluorescence, Microscopy, Staining, Incubation

Journal: Materials Today Bio

Article Title: Ultra-thin elastin-based membranes as an innovative dressing to enhance skin wound healing

doi: 10.1016/j.mtbio.2026.102898

Figure Lengend Snippet: Immunofluorescence staining of fibroblasts and keratinocytes on an ELR-based membrane for 7 and 14 days. A) Expression of multiple markers relevant for fibroblasts. Scale bar = 25 μm. B) Expression of multiple markers pertinent to keratinocytes. Scale bar = 25 μm. C) Gene expression in cells on the VKV-SKS membrane. The expression of VCL, ACTA2 and PTK2 genes in fibroblasts (HFF-1) and D) VCL, KRT14, KRT10, PTK2 and CDH1 genes in keratinocytes (HaCaT) were quantified by qRT-PCR. Gene expression was quantified by qRT-PCR using TaqMan assays, normalized to 18S rRNA as housekeeping genes, and expressed as fold change calculated by the ΔΔCt method. Quantification was performed using n = 3 independent biological replicates per condition. Gene expression data were analysed using one-way ANOVA followed by Dunnett's multiple comparisons test, with day 0 used as the reference. Significance levels reported as follows: (ns (P > 0.05), ∗ (P < 0.05), ∗∗ (P < 0.01), ∗∗∗ (P < 0.001), ∗∗∗∗ (P < 0.0001)).

Article Snippet: Biocompatibility was evaluated using two human cell lines: HFF-1 human foreskin fibroblasts (ATCC® SCRC-1041) and HaCaT immortalized human keratinocytes (CLS-300493).

Techniques: Immunofluorescence, Staining, Membrane, Expressing, Gene Expression, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Cutibacterium acnes -macrophage fusion membrane coating Cu/Zn-MOF for psoriasis treatment via pathological innate lymphoid cells inhibition

doi: 10.1016/j.mtbio.2025.102731

Figure Lengend Snippet: Effects of CM@MOF on HaCaT cells in vitro. (A) Migration ability of HaCaT cells evaluated by scratch assay, violet staining and immunofluorescence staining of Ki67. Green: Ki67; Blue: nuclei (DAPI). Scale bars: 100 μm. (B) Quantitative analysis of cell migration using ImageJ software. (C) Quantitative analysis of cell migrating rate. (D) Quantification of Ki67-positive cells percentage of HaCaT cells. (E) Live (Green)/Dead (Red) staining of HaCaT cells. Scale bars: 100 μm. (F) ROS(Green) in HaCaT cells observed by fluorescence microscopy. Scale bars: 50 μm. (G) Quantitative analysis of the percentage of live HaCaT cells. (H) Quantitative analysis of ROS fluorescence intensity (mean gray value) in HaCaT cells. (I) mRNA levels of IL6, IL17a, IL22, IL23a and Tnfα in HaCaT cells assessed by RT-qPCR. HaCaT: human keratinocyte cells; DAPI: 4′,6-diamidino-2-phenylindole; LPS: Lipopolysaccharide; M2: M2 membrane; CM: Fusion membrane combined with cell wall ( C.acnes ) and membrane (M2-Macrophage); MOF: Metal-organic framework loaded Cu/Zn; CM@MOF: Fusion membrane combined with cell wall ( C.acnes ) and membrane (M2-Macrophage) loaded Cu/Zn metal-organic framework (Cu/Zn-MOF); S. aureus : Staphylococcus aureus ; ROS: reactive oxygen species; RT-qPCR: quantitative Real-Time PCR. Results were presented as the mean ± SD, one way ANOVA was performed for multiple comparison tests, while Student's t -test was performed for two independent groups (ns, no significance, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: Human immortalized keratinocytes (HaCaT; obtained from Cell Lines Service, Eppelheim, #300493) were cultured at 37 °C in a humidified atmosphere with 5 % CO 2 in DMEM supplemented with 10 % FBS.

Techniques: In Vitro, Migration, Wound Healing Assay, Staining, Immunofluorescence, Software, Fluorescence, Microscopy, Quantitative RT-PCR, Membrane, Real-time Polymerase Chain Reaction, Comparison

Journal: ACS Materials Au

Article Title: Lignin-Based Nanoparticles Loaded with Essential Oils: Potential for Diabetic Foot Infection Management

doi: 10.1021/acsmaterialsau.5c00058

Figure Lengend Snippet: Cytocompatibility of EO-loaded QL/CML/TA PEC NPs and free E. citriodora EO toward: a) HaCaT cells and b) BJ-5ta cells, exposed to two concentrations of NPs (0.2 and 0.4 mg/mL) and to two concentrations of EO corresponding to the amount being loaded in the concentrations tested for the NPs for a 24 h period. Results are displayed as mean ± SD from three independent experiments. Samples marked with * show significant differences (* p -value <0.05) when compared to the results obtained for the life control (LC) at 24 h.

Article Snippet: HaCaT immortalized human keratinocyte cell line (300493; Cytion, Eppelheim, Germany) was obtained from Cell Lines Service (CLS, Germany) and provided by DKFZ (Germany).

Techniques: Control